Easy tips for microscope handling

How to use a compound microscope

Know your microscope : know the all part of microscope and their use. Repeat these things until you become familiar.

Move the microscope carefully:

1.     use your both hand , one arm place at arm of microscope and another hand place base of microscope .

2.     hold the microscope close to your body as far as possible.

3.     Put the microscope away from the edge of table

How to focus a histological slide without breaking

1.     Put the slide on the stage and secure by stage clips

2.     At 1^st you use low power lens ×4, just for scanning the slide

3.     Lower the condenser lens

4.     Turn on the light (Illuminator)

5.     Observe where the tissue present in the slide

6.     By moving stage adjustment node you place the tissue at the centre of light seeing from outside ( not adjust this things by placing your eye at optical lens , it kill your time)

7.     Now from outside your lower the objective lens, then place your eye at optical lens and move the objective lens upward

8.     Never lower the objective lens when your eyes are at the optical lens . if you follow this rules you never able to break a slide

Tips for better focusing :

1.     When you use ×4 objective lens , condenser lens should at lower most position and diaphragm should widely open

2.     When you use ×10 objective lens, you move the condenser lens upward and diaphragm should close little bit

3.     When you use ×40 objective lens, you move the condenser lens upward and diaphragm should close little bit

4.     When  you use ×100 objective lens, give oil on the slide and lower the objective lens until it touch the slide , you move the condenser lens upward until it touch the slide and diaphragm should close little more

5.     Before observing the slide one should use the fine adjustment knob , because vision varies person to person

Tips for easy handling of microscope when you observing slide by placing your eye on optical lens

1.     Sit down

2.     After focusing the slide place one hand on fine adjustment knob and another hand on the stage adjustment knob ,

3.     Only these two location you need to handle for  observing  the slide

4.     If you needed to focus the slide again or needed to lower the objective lens you should not place your eye at optical lens at that time , you must see outside and observe until the objective lens touch the slide , than place the eyes on optical lens and move objective lens upward

5.     If the microscope is binocular then adjust the eye pieces according to the distance of your both eye , if the microscope is uniocular then one eye place to eye piece and another one keep open ( don’t close one eye)

Tips for using stage adjustment knob:

                      In microscope you using multiple lens , so you should remember following rules before moving

                       the slide

1.     If you want to move the slide upward you must move the slide downward

2.     If you want to move the slide right you must move the knob to the left and vice versa  

Maintain your microscope :

1. After use turn off the light
2. Remove the slide
3. Change the objective lens to lower power
4. Lower the condenser lens
5. Don’t touch the lens
6. Clean lens by lens paper or lilen cloth
7. Cover the microscope
8. Place the microscope in a dust free location
9. Use silicon in the box of microscope for lowering moistures